murine vsmcs Search Results


96
ATCC murine aortic vsmcs
GFOGER peptide inhibits Pi-induced calcification: (A,B) in smooth muscle cells. Calcification in smooth muscle cells was induced by incubation with 4 mM Pi in absence or presence of GFOGER peptide or GOERFG peptide. <t>(A)</t> <t>MOVAS</t> cells were incubated with two concentrations of GFOGER peptide (250 and 500 μM) or with 500 μM of GOERFG peptide for 10 days. (B) Primary human vascular smooth muscle cells were incubated with 500 μM of GFOGER peptide for 14 days. Calcification was then measured using the OCP method. (C,D) In rat aortic rings. Rat aortic rings were incubated with 4 mM Pi in presence or absence of 500 μM GFOGER peptide for 10 days. (C) Images of one representative Alizarin Red staining experiment are shown. (D) Intracellular calcium content was quantified by OCP colorimetric method. Data are expressed as mean ± SEM of three independent experiments done in triplicate ( n = 3). *** p < 0.001 vs. 4 mM Pi. Parametric one-way ANOVA test.
Murine Aortic Vsmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
ATCC moloney murine leukaemia virus promoter
GFOGER peptide inhibits Pi-induced calcification: (A,B) in smooth muscle cells. Calcification in smooth muscle cells was induced by incubation with 4 mM Pi in absence or presence of GFOGER peptide or GOERFG peptide. <t>(A)</t> <t>MOVAS</t> cells were incubated with two concentrations of GFOGER peptide (250 and 500 μM) or with 500 μM of GOERFG peptide for 10 days. (B) Primary human vascular smooth muscle cells were incubated with 500 μM of GFOGER peptide for 14 days. Calcification was then measured using the OCP method. (C,D) In rat aortic rings. Rat aortic rings were incubated with 4 mM Pi in presence or absence of 500 μM GFOGER peptide for 10 days. (C) Images of one representative Alizarin Red staining experiment are shown. (D) Intracellular calcium content was quantified by OCP colorimetric method. Data are expressed as mean ± SEM of three independent experiments done in triplicate ( n = 3). *** p < 0.001 vs. 4 mM Pi. Parametric one-way ANOVA test.
Moloney Murine Leukaemia Virus Promoter, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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moloney murine leukaemia virus promoter - by Bioz Stars, 2026-03
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90
Jackson Laboratory primary murine aortic smooth muscle cells
GFOGER peptide inhibits Pi-induced calcification: (A,B) in smooth muscle cells. Calcification in smooth muscle cells was induced by incubation with 4 mM Pi in absence or presence of GFOGER peptide or GOERFG peptide. <t>(A)</t> <t>MOVAS</t> cells were incubated with two concentrations of GFOGER peptide (250 and 500 μM) or with 500 μM of GOERFG peptide for 10 days. (B) Primary human vascular smooth muscle cells were incubated with 500 μM of GFOGER peptide for 14 days. Calcification was then measured using the OCP method. (C,D) In rat aortic rings. Rat aortic rings were incubated with 4 mM Pi in presence or absence of 500 μM GFOGER peptide for 10 days. (C) Images of one representative Alizarin Red staining experiment are shown. (D) Intracellular calcium content was quantified by OCP colorimetric method. Data are expressed as mean ± SEM of three independent experiments done in triplicate ( n = 3). *** p < 0.001 vs. 4 mM Pi. Parametric one-way ANOVA test.
Primary Murine Aortic Smooth Muscle Cells, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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97
ATCC murine vascular smooth muscle sv40lt smc cells vsmcs
GFOGER peptide inhibits Pi-induced calcification: (A,B) in smooth muscle cells. Calcification in smooth muscle cells was induced by incubation with 4 mM Pi in absence or presence of GFOGER peptide or GOERFG peptide. <t>(A)</t> <t>MOVAS</t> cells were incubated with two concentrations of GFOGER peptide (250 and 500 μM) or with 500 μM of GOERFG peptide for 10 days. (B) Primary human vascular smooth muscle cells were incubated with 500 μM of GFOGER peptide for 14 days. Calcification was then measured using the OCP method. (C,D) In rat aortic rings. Rat aortic rings were incubated with 4 mM Pi in presence or absence of 500 μM GFOGER peptide for 10 days. (C) Images of one representative Alizarin Red staining experiment are shown. (D) Intracellular calcium content was quantified by OCP colorimetric method. Data are expressed as mean ± SEM of three independent experiments done in triplicate ( n = 3). *** p < 0.001 vs. 4 mM Pi. Parametric one-way ANOVA test.
Murine Vascular Smooth Muscle Sv40lt Smc Cells Vsmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
ScienCell primary murine vascular smooth muscle cells
GFOGER peptide inhibits Pi-induced calcification: (A,B) in smooth muscle cells. Calcification in smooth muscle cells was induced by incubation with 4 mM Pi in absence or presence of GFOGER peptide or GOERFG peptide. <t>(A)</t> <t>MOVAS</t> cells were incubated with two concentrations of GFOGER peptide (250 and 500 μM) or with 500 μM of GOERFG peptide for 10 days. (B) Primary human vascular smooth muscle cells were incubated with 500 μM of GFOGER peptide for 14 days. Calcification was then measured using the OCP method. (C,D) In rat aortic rings. Rat aortic rings were incubated with 4 mM Pi in presence or absence of 500 μM GFOGER peptide for 10 days. (C) Images of one representative Alizarin Red staining experiment are shown. (D) Intracellular calcium content was quantified by OCP colorimetric method. Data are expressed as mean ± SEM of three independent experiments done in triplicate ( n = 3). *** p < 0.001 vs. 4 mM Pi. Parametric one-way ANOVA test.
Primary Murine Vascular Smooth Muscle Cells, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Jackson Laboratory wild-type murine primary vascular smooth muscle cells
Vascular smooth muscle cells (panel A ) and C6 glioma cells (panel B ) were pretreated with domain specific PAs or wild type Thioredoxin and TSc controls, and stimulated with S100B. Cell lysates were separated by SDS-PAGE and immunobloted with antibodies specific for phospho-ERK1/ERK2 (upper gel panels) or total ERK1/ERK2 (bottom gel panels). Images were digitized and results represented on the bar diagrams. Note the marked inhibition of ERK phosphorylation in the presence of anti-RAGE PAs. The data are representative of three separate experiments.
Wild Type Murine Primary Vascular Smooth Muscle Cells, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Charles River Laboratories murine vsmc
Vascular smooth muscle cells (panel A ) and C6 glioma cells (panel B ) were pretreated with domain specific PAs or wild type Thioredoxin and TSc controls, and stimulated with S100B. Cell lysates were separated by SDS-PAGE and immunobloted with antibodies specific for phospho-ERK1/ERK2 (upper gel panels) or total ERK1/ERK2 (bottom gel panels). Images were digitized and results represented on the bar diagrams. Note the marked inhibition of ERK phosphorylation in the presence of anti-RAGE PAs. The data are representative of three separate experiments.
Murine Vsmc, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


GFOGER peptide inhibits Pi-induced calcification: (A,B) in smooth muscle cells. Calcification in smooth muscle cells was induced by incubation with 4 mM Pi in absence or presence of GFOGER peptide or GOERFG peptide. (A) MOVAS cells were incubated with two concentrations of GFOGER peptide (250 and 500 μM) or with 500 μM of GOERFG peptide for 10 days. (B) Primary human vascular smooth muscle cells were incubated with 500 μM of GFOGER peptide for 14 days. Calcification was then measured using the OCP method. (C,D) In rat aortic rings. Rat aortic rings were incubated with 4 mM Pi in presence or absence of 500 μM GFOGER peptide for 10 days. (C) Images of one representative Alizarin Red staining experiment are shown. (D) Intracellular calcium content was quantified by OCP colorimetric method. Data are expressed as mean ± SEM of three independent experiments done in triplicate ( n = 3). *** p < 0.001 vs. 4 mM Pi. Parametric one-way ANOVA test.

Journal: Frontiers in Cell and Developmental Biology

Article Title: GFOGER Peptide Modifies the Protein Content of Extracellular Vesicles and Inhibits Vascular Calcification

doi: 10.3389/fcell.2020.589761

Figure Lengend Snippet: GFOGER peptide inhibits Pi-induced calcification: (A,B) in smooth muscle cells. Calcification in smooth muscle cells was induced by incubation with 4 mM Pi in absence or presence of GFOGER peptide or GOERFG peptide. (A) MOVAS cells were incubated with two concentrations of GFOGER peptide (250 and 500 μM) or with 500 μM of GOERFG peptide for 10 days. (B) Primary human vascular smooth muscle cells were incubated with 500 μM of GFOGER peptide for 14 days. Calcification was then measured using the OCP method. (C,D) In rat aortic rings. Rat aortic rings were incubated with 4 mM Pi in presence or absence of 500 μM GFOGER peptide for 10 days. (C) Images of one representative Alizarin Red staining experiment are shown. (D) Intracellular calcium content was quantified by OCP colorimetric method. Data are expressed as mean ± SEM of three independent experiments done in triplicate ( n = 3). *** p < 0.001 vs. 4 mM Pi. Parametric one-way ANOVA test.

Article Snippet: Murine aortic VSMCs (MOVAS-1 CRL-2797, ATCC, Manassas, VA, United States) were maintained in DMEM 6546 medium supplemented with 10% FCS, 100 IU/ml penicillin, 100 μg/ml streptomycin, 4 mM glutamine, and 200 μg/ml geneticin (G418) at 37°C in a humidified 5% CO 2 atmosphere.

Techniques: Incubation, Staining

Vascular smooth muscle cells (panel A ) and C6 glioma cells (panel B ) were pretreated with domain specific PAs or wild type Thioredoxin and TSc controls, and stimulated with S100B. Cell lysates were separated by SDS-PAGE and immunobloted with antibodies specific for phospho-ERK1/ERK2 (upper gel panels) or total ERK1/ERK2 (bottom gel panels). Images were digitized and results represented on the bar diagrams. Note the marked inhibition of ERK phosphorylation in the presence of anti-RAGE PAs. The data are representative of three separate experiments.

Journal: PLoS ONE

Article Title: Combinatorial Library of Improved Peptide Aptamers, CLIPs to Inhibit RAGE Signal Transduction in Mammalian Cells

doi: 10.1371/journal.pone.0065180

Figure Lengend Snippet: Vascular smooth muscle cells (panel A ) and C6 glioma cells (panel B ) were pretreated with domain specific PAs or wild type Thioredoxin and TSc controls, and stimulated with S100B. Cell lysates were separated by SDS-PAGE and immunobloted with antibodies specific for phospho-ERK1/ERK2 (upper gel panels) or total ERK1/ERK2 (bottom gel panels). Images were digitized and results represented on the bar diagrams. Note the marked inhibition of ERK phosphorylation in the presence of anti-RAGE PAs. The data are representative of three separate experiments.

Article Snippet: Wild-type murine primary vascular smooth muscle cells were isolated and cultured from the aortas of 10-week-old male C57BL/6 mice (The Jackson Laboratory) using a modification of the procedure of Tarvo and Barret .

Techniques: SDS Page, Inhibition, Phospho-proteomics